P0711S, Rapid PNGase F (non-reducing format) - 50 reactions

Developed for proteomic applications, Rapid PNGase F (non-reducing format) enables complete and rapid deglycosylation, while preserving disulfide bonds. This facilitates high throughput proteomics applications and methods for antibody characterization by mass spectrometry, such as intact mass analysis. Rapid PNGase F (non-reducing format) combines the advantages of Rapid PNGase F (fast processing time), with non-reducing conditions, preserving quaternary structure.

• Complete deglycosylation of antibodies and immunoglobulin fusion proteins in 15 minutes
• Recombinant enzyme; guaranteed endoglycosidase F1, F2 or F3 free
• All reaction components are compatible with HPLC and mass spectrometry analysis
• ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
• Optimal activity and stability for up to 12 months
• Fast reaction setup

Specificity

Rapid PNGase F (non-reducing format) cleaves all complex, hybrid and high-mannose type glycans from antibodies and related proteins. Core α1,3 fucosylation (found in immunoglobulins expressed in plant or insect cells) is resistant to both Rapid PNGase F and PNGase F.