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Oligonucleotides can be modified by direct incorporation during the synthesis or by post-synthesis labelling.

Direct incorporation


3’ modifications 

Since automated oligonucleotide synthesis is realised from 3’ to 5’, these modifications are only possible if the corresponding solid support (CPG column) is available and if the modification is compatible with the chemistries used during the synthesis. Typical examples are 3’-phosphate, 3’ Biotin, 3’ FAM, 3’ DDQ I, 3’ BHQ-1R…


5’ and internal modifications

Many modifications can be directly introduced at the 5’end or at internal positions of the oligonucleotides using the phosphoramidites. However these modifications need to support the somewhat harsh cleavage-deprotection conditions including a strong basic pH. Typical examples are 5’ Biotin, 5’ Phosphate, 5’ Cholesterol, 5’ FAM, 8-Oxo-dA,Biotin-dT, DABCYL-dT…

Post-synthesis incorporation

Post-synthesis modifications may influence the yield of the reaction. A lower yield may result from poly-modifications and/or strong secondary structures. Two major post-synthesis reactions are used to introduce sensitive dyes or compounds that do not exist as phosphoramidites. In the first case the label is conjugated to an amino-modified oligonucleotide (3’, 5’ or on a dT) using its amino-reactive version (N-hydroxysuccinimide (NHS) ester in most cases). The second possibility (originally also used for synthesis of molecular beacons) is the addition of a maleimide-modified label to a thiol-modified oligonucleotide. 

Label conjugated to an amino-modified oligonucleotide
Label conjugated to an amino-modified oligonucleotide

Maleimide-modified label to a thiol-modified oligonucleotide.
Maleimide-modified label to a thiol-modified oligonucleotide.

Oligonucleotides modification classification
Click to enlarge
• Post-synthesis modifications are highlighted in blue.
• Some modifications can be inserted after or during the synthesis and are in red. 
• To know the best purification according to each modification, consult the oligonucleotides purification table



Discover the Dyes compatibility tables

Modifications

Setați descendent

Articolele 1-100 din 710

Pagină
pe pagină
  1. 2' Fluoro RNA A 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB201-IN100
    TVA inclus
    285,60 RON
  2. 2' Fluoro RNA A 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB201-IN020
    TVA inclus
    214,20 RON
  3. 2' Fluoro RNA A 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB201-IN004
    TVA inclus
    199,92 RON
  4. 2' Fluoro RNA C 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB202-IN100
    TVA inclus
    285,60 RON
  5. 2' Fluoro RNA C 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB202-IN020
    TVA inclus
    214,20 RON
  6. 2' Fluoro RNA C 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB202-IN004
    TVA inclus
    199,92 RON
  7. 2' Fluoro RNA G 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB203-IN100
    TVA inclus
    285,60 RON
  8. 2' Fluoro RNA G 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB203-IN020
    TVA inclus
    214,20 RON
  9. 2' Fluoro RNA G 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB203-IN004
    TVA inclus
    199,92 RON
  10. 2' Fluoro RNA U 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB208-IN100
    TVA inclus
    285,60 RON
  11. 2' Fluoro RNA U 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB208-IN020
    TVA inclus
    214,20 RON
  12. 2' Fluoro RNA U 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB208-IN004
    TVA inclus
    199,92 RON
  13. 2' O-Me RNA Inosine 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB210-IN100
    TVA inclus
    464,10 RON
  14. 2' O-Me RNA Inosine 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB210-IN020
    TVA inclus
    321,30 RON
  15. 2' O-Me RNA Inosine 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB210-IN004
    TVA inclus
    285,60 RON
  16. 2' O-Me RNA Propynyl-C 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB232-IN100
    TVA inclus
    11.899.998,81 RON
  17. 2' O-Me RNA Propynyl-C 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB232-IN020
    TVA inclus
    11.899.998,81 RON
  18. 2' O-Me RNA Propynyl-C 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB232-IN004
    TVA inclus
    11.899.998,81 RON
  19. 2' O-Me RNA Propynyl-U 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB238-IN100
    TVA inclus
    11.899.998,81 RON
  20. 2' O-Me RNA Propynyl-U 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB238-IN020
    TVA inclus
    11.899.998,81 RON
  21. 2' O-Me RNA Propynyl-U 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB238-IN004
    TVA inclus
    11.899.998,81 RON
  22. 2' O-Me RNA-5-Me-C 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB222-IN100
    TVA inclus
    928,20 RON
  23. 2' O-Me RNA-5-Me-C 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB222-IN020
    TVA inclus
    535,50 RON
  24. 2' O-Me RNA-5-Me-C 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB222-IN004
    TVA inclus
    428,40 RON
  25. 2', 3' ddC Blocked 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB080-03100
    TVA inclus
    1.285,20 RON
  26. 2', 3' ddC Blocked 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB080-03020
    TVA inclus
    856,80 RON
  27. 2', 3' ddC Blocked 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB080-03004
    TVA inclus
    714,00 RON
  28. 2-Amino-dA NEW 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB160-IN100
    TVA inclus
    1.178,10 RON
  29. 2-Amino-dA NEW 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB160-IN020
    TVA inclus
    785,40 RON
  30. 2-Amino-dA NEW 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB160-IN004
    TVA inclus
    692,58 RON
  31. 2-Aminopurine (DNA) 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB170-IN100
    TVA inclus
    1.178,10 RON
  32. 2-Aminopurine (DNA) 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB170-IN020
    TVA inclus
    785,40 RON
  33. 2-Aminopurine (DNA) 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB170-IN004
    TVA inclus
    599,76 RON
  34. 3 Phosphate 2,5 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-03M02
    TVA inclus
    749,70 RON
  35. 3' (5-Br-dU) 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB098-03100
    TVA inclus
    1.071,00 RON
  36. 3' (5-Br-dU) 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB098-03020
    TVA inclus
    671,16 RON
  37. 3' (5-Br-dU) 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB098-03004
    TVA inclus
    399,84 RON
  38. 3' (5-Me-dC) 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB152-03100
    TVA inclus
    721,14 RON
  39. 3' (5-Me-dC) 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB152-03020
    TVA inclus
    464,10 RON
  40. 3' (5-Me-dC) 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB152-03004
    TVA inclus
    392,70 RON
  41. 3' 6-FAM 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL001-03100
    TVA inclus
    706,86 RON
  42. 3' 6-FAM 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL001-03020
    TVA inclus
    421,26 RON
  43. 3' 6-FAM 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL001-03004
    TVA inclus
    349,86 RON
  44. 3' Amino Modifier C6 10 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-MF006-03M10
    TVA inclus
    2.142,00 RON
  45. 3' Amino Modifier C6 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-MF006-03100
    TVA inclus
    392,70 RON
  46. 3' Amino Modifier C6 2,5 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-MF006-03M02
    TVA inclus
    714,00 RON
  47. 3' Amino Modifier C6 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-MF006-03020
    TVA inclus
    285,60 RON
  48. 3' Amino Modifier C6 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-MF006-03004
    TVA inclus
    214,20 RON
  49. 3' Amino Modifier C6 5 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-MF006-03M05
    TVA inclus
    1.356,60 RON
  50. 3' BHQ-1™ / BHQ-2™ / BHQ-3™ 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-03100
    TVA inclus
    1.035,30 RON
  51. 3' BHQ-1™ / BHQ-2™ / BHQ-3™ 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-03020
    TVA inclus
    649,74 RON
  52. 3' BHQ-1™ / BHQ-2™ / BHQ-3™ 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-03004
    TVA inclus
    492,66 RON
  53. 3' Biotin C3 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-BT030-03100
    TVA inclus
    535,50 RON
  54. 3' Biotin C3 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-BT030-03020
    TVA inclus
    349,86 RON
  55. 3' Biotin C3 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-BT030-03004
    TVA inclus
    321,30 RON
  56. 3' Biotin TEG 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-BT001-03100
    TVA inclus
    606,90 RON
  57. 3' Biotin TEG 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-BT001-03020
    TVA inclus
    428,40 RON
  58. 3' Biotin TEG 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-BT001-03004
    TVA inclus
    357,00 RON
  59. 3' Cascade Blue® 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL070-CBL03100
    TVA inclus
    11.899.998,81 RON
  60. 3' Cascade Blue® 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL070-CBL03020
    TVA inclus
    11.899.998,81 RON
  61. 3' Cascade Blue® 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL070-CBL03004
    TVA inclus
    11.899.998,81 RON
  62. 3' Cy5® 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL055-03100
    TVA inclus
    2.499,00 RON
  63. 3' Cy5® 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL055-03020
    TVA inclus
    1.642,20 RON
  64. 3' Cy5® 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL055-03004
    TVA inclus
    1.213,80 RON
  65. 3' Cy® 3 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL050-03100
    TVA inclus
    2.427,60 RON
  66. 3' Cy® 3 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL050-03020
    TVA inclus
    1.570,80 RON
  67. 3' Cy® 3 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL050-03004
    TVA inclus
    1.142,40 RON
  68. 3' Cy® 3.5 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL053-03100
    TVA inclus
    11.899.998,81 RON
  69. 3' Cy® 3.5 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL053-03020
    TVA inclus
    11.899.998,81 RON
  70. 3' Cy® 3.5 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL053-03004
    TVA inclus
    11.899.998,81 RON
  71. 3' Cy® 5.5 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL057-03100
    TVA inclus
    11.899.998,81 RON
  72. 3' Cy® 5.5 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL057-03020
    TVA inclus
    11.899.998,81 RON
  73. 3' Cy® 5.5 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL057-03004
    TVA inclus
    11.899.998,81 RON
  74. 3' dA Blocked 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB070-03100
    TVA inclus
    1.499,40 RON
  75. 3' dA Blocked 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB070-03020
    TVA inclus
    785,40 RON
  76. 3' dA Blocked 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB070-03004
    TVA inclus
    499,80 RON
  77. 3' DABCYL 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-03100
    TVA inclus
    821,10 RON
  78. 3' DABCYL 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-03020
    TVA inclus
    571,20 RON
  79. 3' DABCYL 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-03004
    TVA inclus
    464,10 RON
  80. 3' dC Blocked 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB072-03100
    TVA inclus
    1.213,80 RON
  81. 3' dC Blocked 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB072-03020
    TVA inclus
    785,40 RON
  82. 3' dC Blocked 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB072-03004
    TVA inclus
    499,80 RON
  83. 3' Deep Dark Quencher I 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU400-03100
    TVA inclus
    1.071,00 RON
  84. 3' Deep Dark Quencher I 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU400-03020
    TVA inclus
    678,30 RON
  85. 3' Deep Dark Quencher I 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU400-03004
    TVA inclus
    535,50 RON
  86. 3' Deep Dark Quencher II 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-03100
    TVA inclus
    1.642,20 RON
  87. 3' Deep Dark Quencher II 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-03020
    TVA inclus
    778,26 RON
  88. 3' Deep Dark Quencher II 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-03004
    TVA inclus
    499,80 RON
  89. 3' dG Blocked 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB074-03100
    TVA inclus
    1.213,80 RON
  90. 3' dG Blocked 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB074-03020
    TVA inclus
    785,40 RON
  91. 3' dG Blocked 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB074-03004
    TVA inclus
    499,80 RON
  92. 3' Digoxigenin 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-CN001-03100
    TVA inclus
    992,46 RON
  93. 3' Digoxigenin 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-CN001-03020
    TVA inclus
    706,86 RON
  94. 3' Digoxigenin 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-CN001-03004
    TVA inclus
    635,46 RON
  95. 3' dT Blocked 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB076-03100
    TVA inclus
    1.213,80 RON
  96. 3' dT Blocked 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB076-03020
    TVA inclus
    785,40 RON
  97. 3' dT Blocked 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB076-03004
    TVA inclus
    499,80 RON
  98. 3' dUracil 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB068-03100
    TVA inclus
    321,30 RON
  99. 3' dUracil 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB068-03020
    TVA inclus
    257,04 RON
  100. 3' dUracil 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB068-03004
    TVA inclus
    228,48 RON
Setați descendent

Articolele 1-100 din 710

Pagină
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Modifications
  1. Conjugates
  2. Degenerate bases
  3. Fluorescent dyes
  4. Modifiers
  5. Non natural bases
  6. Phosphates
  7. Quenchers
  8. Spacers
  9. Biotins
  10. NGS Modifications
  11. Purifications