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Oligonucleotides can be modified by direct incorporation during the synthesis or by post-synthesis labelling.

Direct incorporation


3’ modifications 

Since automated oligonucleotide synthesis is realised from 3’ to 5’, these modifications are only possible if the corresponding solid support (CPG column) is available and if the modification is compatible with the chemistries used during the synthesis. Typical examples are 3’-phosphate, 3’ Biotin, 3’ FAM, 3’ DDQ I, 3’ BHQ-1R…


5’ and internal modifications

Many modifications can be directly introduced at the 5’end or at internal positions of the oligonucleotides using the phosphoramidites. However these modifications need to support the somewhat harsh cleavage-deprotection conditions including a strong basic pH. Typical examples are 5’ Biotin, 5’ Phosphate, 5’ Cholesterol, 5’ FAM, 8-Oxo-dA,Biotin-dT, DABCYL-dT…

Post-synthesis incorporation

Post-synthesis modifications may influence the yield of the reaction. A lower yield may result from poly-modifications and/or strong secondary structures. Two major post-synthesis reactions are used to introduce sensitive dyes or compounds that do not exist as phosphoramidites. In the first case the label is conjugated to an amino-modified oligonucleotide (3’, 5’ or on a dT) using its amino-reactive version (N-hydroxysuccinimide (NHS) ester in most cases). The second possibility (originally also used for synthesis of molecular beacons) is the addition of a maleimide-modified label to a thiol-modified oligonucleotide. 

Label conjugated to an amino-modified oligonucleotide
Label conjugated to an amino-modified oligonucleotide

Maleimide-modified label to a thiol-modified oligonucleotide.
Maleimide-modified label to a thiol-modified oligonucleotide.

Oligonucleotides modification classification
Click to enlarge
• Post-synthesis modifications are highlighted in blue.
• Some modifications can be inserted after or during the synthesis and are in red. 
• To know the best purification according to each modification, consult the oligonucleotides purification table



Discover the Dyes compatibility tables

Modifications

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Articolele 1-15 din 710

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  1. 5' Cy® 3.5 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL053-05004
    TVA inclus
    749,70 RON
  2. Spacer 9 / TEG 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP009-IN004
    TVA inclus
    249,90 RON
  3. Spacer C8 (Octanediol) 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP008-IN100
    TVA inclus
    11.899.998,81 RON
  4. Spacer C8 (Octanediol) 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP008-IN020
    TVA inclus
    11.899.998,81 RON
  5. Spacer C8 (Octanediol) 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP008-IN004
    TVA inclus
    11.899.998,81 RON
  6. Spacer C3 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-IN100
    TVA inclus
    606,90 RON
  7. Spacer C3 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-IN020
    TVA inclus
    392,70 RON
  8. Spacer C3 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-IN004
    TVA inclus
    285,60 RON
  9. 3' Spacer C3 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-03100
    TVA inclus
    606,90 RON
  10. 3' Spacer C3 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-03020
    TVA inclus
    392,70 RON
  11. 3' Spacer C3 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-03004
    TVA inclus
    285,60 RON
  12. 3' QXLTM 490/570/610/670/680 NEW 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU700-03100
    TVA inclus
    749,70 RON
  13. 3' QXLTM 490/570/610/670/680 NEW 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU700-03020
    TVA inclus
    499,80 RON
  14. 3' QXLTM 490/570/610/670/680 NEW 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU700-03004
    TVA inclus
    392,70 RON
  15. BHQ-1™/BHQ-2™/BHQ-3™ (dT) 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-DT100
    TVA inclus
    1.785,00 RON
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Articolele 1-15 din 710

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Modifications
  1. Conjugates
  2. Degenerate bases
  3. Fluorescent dyes
  4. Modifiers
  5. Non natural bases
  6. Phosphates
  7. Quenchers
  8. Spacers
  9. Biotins
  10. NGS Modifications
  11. Purifications