Monedă
RON
Limba
Romanian
  • Comparați ()
  • Contactați-ne
  • Conectare
  • Creați cont
Cart 0
Comutare în navigare
Căutare
Căutare avansată
SUNA ACUM
+40 (264) 523 281
Menu
Account
Setări
Limba
Romanian
Monedă
RON

Oligonucleotides can be modified by direct incorporation during the synthesis or by post-synthesis labelling.

Direct incorporation


3’ modifications 

Since automated oligonucleotide synthesis is realised from 3’ to 5’, these modifications are only possible if the corresponding solid support (CPG column) is available and if the modification is compatible with the chemistries used during the synthesis. Typical examples are 3’-phosphate, 3’ Biotin, 3’ FAM, 3’ DDQ I, 3’ BHQ-1R…


5’ and internal modifications

Many modifications can be directly introduced at the 5’end or at internal positions of the oligonucleotides using the phosphoramidites. However these modifications need to support the somewhat harsh cleavage-deprotection conditions including a strong basic pH. Typical examples are 5’ Biotin, 5’ Phosphate, 5’ Cholesterol, 5’ FAM, 8-Oxo-dA,Biotin-dT, DABCYL-dT…

Post-synthesis incorporation

Post-synthesis modifications may influence the yield of the reaction. A lower yield may result from poly-modifications and/or strong secondary structures. Two major post-synthesis reactions are used to introduce sensitive dyes or compounds that do not exist as phosphoramidites. In the first case the label is conjugated to an amino-modified oligonucleotide (3’, 5’ or on a dT) using its amino-reactive version (N-hydroxysuccinimide (NHS) ester in most cases). The second possibility (originally also used for synthesis of molecular beacons) is the addition of a maleimide-modified label to a thiol-modified oligonucleotide. 

Label conjugated to an amino-modified oligonucleotide
Label conjugated to an amino-modified oligonucleotide

Maleimide-modified label to a thiol-modified oligonucleotide.
Maleimide-modified label to a thiol-modified oligonucleotide.

Oligonucleotides modification classification
Click to enlarge
• Post-synthesis modifications are highlighted in blue.
• Some modifications can be inserted after or during the synthesis and are in red. 
• To know the best purification according to each modification, consult the oligonucleotides purification table



Discover the Dyes compatibility tables

Modifications

Setați descendent

Articolele 1-100 din 710

Pagină
pe pagină
  1. 5' Cy® 3.5 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-FL053-05004
    TVA inclus
    749,70 RON
  2. Spacer 9 / TEG 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP009-IN004
    TVA inclus
    249,90 RON
  3. Spacer C8 (Octanediol) 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP008-IN100
    TVA inclus
    11.899.998,81 RON
  4. Spacer C8 (Octanediol) 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP008-IN020
    TVA inclus
    11.899.998,81 RON
  5. Spacer C8 (Octanediol) 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP008-IN004
    TVA inclus
    11.899.998,81 RON
  6. Spacer C3 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-IN100
    TVA inclus
    606,90 RON
  7. Spacer C3 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-IN020
    TVA inclus
    392,70 RON
  8. Spacer C3 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-IN004
    TVA inclus
    285,60 RON
  9. 3' Spacer C3 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-03100
    TVA inclus
    606,90 RON
  10. 3' Spacer C3 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-03020
    TVA inclus
    392,70 RON
  11. 3' Spacer C3 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-SP003-03004
    TVA inclus
    285,60 RON
  12. 3' QXLTM 490/570/610/670/680 NEW 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU700-03100
    TVA inclus
    749,70 RON
  13. 3' QXLTM 490/570/610/670/680 NEW 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU700-03020
    TVA inclus
    499,80 RON
  14. 3' QXLTM 490/570/610/670/680 NEW 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU700-03004
    TVA inclus
    392,70 RON
  15. BHQ-1™/BHQ-2™/BHQ-3™ (dT) 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-DT100
    TVA inclus
    1.785,00 RON
  16. BHQ-1™/BHQ-2™/BHQ-3™ (dT) 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-DT020
    TVA inclus
    1.356,60 RON
  17. BHQ-1™/BHQ-2™(dT) 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-DT004
    TVA inclus
    999,60 RON
  18. 5' BHQ-1™ / BHQ-2™ 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-05100
    TVA inclus
    1.285,20 RON
  19. 5' BHQ-1™ / BHQ-2™ 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-05020
    TVA inclus
    821,10 RON
  20. 5' BHQ-1™ / BHQ-2™ 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-05004
    TVA inclus
    635,46 RON
  21. 3' BHQ-1™ / BHQ-2™ / BHQ-3™ 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-03100
    TVA inclus
    1.035,30 RON
  22. 3' BHQ-1™ / BHQ-2™ / BHQ-3™ 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-03020
    TVA inclus
    649,74 RON
  23. 3' BHQ-1™ / BHQ-2™ / BHQ-3™ 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU600-03004
    TVA inclus
    492,66 RON
  24. Deep Dark Quencher II (dR) 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-DR100
    TVA inclus
    11.899.998,81 RON
  25. Deep Dark Quencher II (dR) 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-DR020
    TVA inclus
    11.899.998,81 RON
  26. Deep Dark Quencher II (dR) 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-DR004
    TVA inclus
    11.899.998,81 RON
  27. 5' Deep Dark Quencher II 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-05100
    TVA inclus
    1.642,20 RON
  28. 5' Deep Dark Quencher II 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-05020
    TVA inclus
    999,60 RON
  29. 5' Deep Dark Quencher II 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-05004
    TVA inclus
    714,00 RON
  30. 3' Deep Dark Quencher II 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-03100
    TVA inclus
    1.642,20 RON
  31. 3' Deep Dark Quencher II 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-03020
    TVA inclus
    778,26 RON
  32. 3' Deep Dark Quencher II 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU500-03004
    TVA inclus
    499,80 RON
  33. Deep Dark Quencher I (dR) 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU400-DR100
    TVA inclus
    11.899.998,81 RON
  34. Deep Dark Quencher I (dR) 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU400-DR020
    TVA inclus
    11.899.998,81 RON
  35. Deep Dark Quencher I (dR) 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU400-DR004
    TVA inclus
    11.899.998,81 RON
  36. 3' Deep Dark Quencher I 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU400-03100
    TVA inclus
    1.071,00 RON
  37. 3' Deep Dark Quencher I 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU400-03020
    TVA inclus
    678,30 RON
  38. 3' Deep Dark Quencher I 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU400-03004
    TVA inclus
    535,50 RON
  39. 5' Eclipse® Dark Quencher 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU300-05100
    TVA inclus
    1.499,40 RON
  40. 5' Eclipse® Dark Quencher 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU300-05020
    TVA inclus
    928,20 RON
  41. 5' Eclipse® Dark Quencher 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU300-05004
    TVA inclus
    678,30 RON
  42. 3' Eclipse® Dark Quencher 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU300-03100
    TVA inclus
    1.071,00 RON
  43. 3' Eclipse® Dark Quencher 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU300-03020
    TVA inclus
    678,30 RON
  44. 3' Eclipse® Dark Quencher 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU300-03004
    TVA inclus
    535,50 RON
  45. DABCYL (dT) 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-DT100
    TVA inclus
    1.420,86 RON
  46. DABCYL (dT) 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-DT020
    TVA inclus
    892,50 RON
  47. DABCYL (dT) 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-DT004
    TVA inclus
    714,00 RON
  48. 5' DABCYL 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-05100
    TVA inclus
    1.142,40 RON
  49. 5' DABCYL 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-05020
    TVA inclus
    714,00 RON
  50. 5' DABCYL 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-05004
    TVA inclus
    571,20 RON
  51. 3' DABCYL 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-03100
    TVA inclus
    821,10 RON
  52. 3' DABCYL 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-03020
    TVA inclus
    571,20 RON
  53. 3' DABCYL 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU200-03004
    TVA inclus
    464,10 RON
  54. TAMRA (dT) 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU100-DT100
    TVA inclus
    1.285,20 RON
  55. TAMRA (dT) 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU100-DT020
    TVA inclus
    999,60 RON
  56. TAMRA (dT) 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU100-DT004
    TVA inclus
    785,40 RON
  57. 3' TAMRA 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU100-03100
    TVA inclus
    821,10 RON
  58. 3' TAMRA 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU100-03020
    TVA inclus
    535,50 RON
  59. 3' TAMRA 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-QU100-03004
    TVA inclus
    392,70 RON
  60. 5' Phosphate 20 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-05M20
    TVA inclus
    1.320,90 RON
  61. 5' Phosphate 10 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-05M10
    TVA inclus
    892,50 RON
  62. 5' Phosphate 5 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-05M05
    TVA inclus
    606,90 RON
  63. 5' Phosphate 2,5 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-05M02
    TVA inclus
    464,10 RON
  64. 5' Phosphate 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-05100
    TVA inclus
    285,60 RON
  65. 5' Phosphate 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-05020
    TVA inclus
    214,20 RON
  66. 5' Phosphate 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-05004
    TVA inclus
    178,50 RON
  67. 3' Phosphate 20 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-03M20
    TVA inclus
    3.141,60 RON
  68. 3' Phosphate 10 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-03M10
    TVA inclus
    2.213,40 RON
  69. 3' Phosphate 5 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-03M05
    TVA inclus
    1.142,40 RON
  70. 3 Phosphate 2,5 µmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-03M02
    TVA inclus
    749,70 RON
  71. 3' Phosphate 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-03100
    TVA inclus
    428,40 RON
  72. 3' Phosphate 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-03020
    TVA inclus
    249,90 RON
  73. 3' Phosphate 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-PH001-03004
    TVA inclus
    214,20 RON
  74. 2' O-Me RNA Propynyl-U 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB238-IN100
    TVA inclus
    11.899.998,81 RON
  75. 2' O-Me RNA Propynyl-U 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB238-IN020
    TVA inclus
    11.899.998,81 RON
  76. 2' O-Me RNA Propynyl-U 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB238-IN004
    TVA inclus
    11.899.998,81 RON
  77. 2' O-Me RNA Propynyl-C 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB232-IN100
    TVA inclus
    11.899.998,81 RON
  78. 2' O-Me RNA Propynyl-C 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB232-IN020
    TVA inclus
    11.899.998,81 RON
  79. 2' O-Me RNA Propynyl-C 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB232-IN004
    TVA inclus
    11.899.998,81 RON
  80. 2' O-Me RNA-5-Me-C 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB222-IN100
    TVA inclus
    928,20 RON
  81. 2' O-Me RNA-5-Me-C 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB222-IN020
    TVA inclus
    535,50 RON
  82. 2' O-Me RNA-5-Me-C 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB222-IN004
    TVA inclus
    428,40 RON
  83. 2' O-Me RNA Inosine 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB210-IN100
    TVA inclus
    464,10 RON
  84. 2' O-Me RNA Inosine 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB210-IN020
    TVA inclus
    321,30 RON
  85. 2' O-Me RNA Inosine 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB210-IN004
    TVA inclus
    285,60 RON
  86. 2' Fluoro RNA U 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB208-IN100
    TVA inclus
    285,60 RON
  87. 2' Fluoro RNA U 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB208-IN020
    TVA inclus
    214,20 RON
  88. 2' Fluoro RNA U 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB208-IN004
    TVA inclus
    199,92 RON
  89. 2' Fluoro RNA G 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB203-IN100
    TVA inclus
    285,60 RON
  90. 2' Fluoro RNA G 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB203-IN020
    TVA inclus
    214,20 RON
  91. 2' Fluoro RNA G 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB203-IN004
    TVA inclus
    199,92 RON
  92. 2' Fluoro RNA C 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB202-IN100
    TVA inclus
    285,60 RON
  93. 2' Fluoro RNA C 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB202-IN020
    TVA inclus
    214,20 RON
  94. 2' Fluoro RNA C 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB202-IN004
    TVA inclus
    199,92 RON
  95. 2' Fluoro RNA A 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB201-IN100
    TVA inclus
    285,60 RON
  96. 2' Fluoro RNA A 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB201-IN020
    TVA inclus
    214,20 RON
  97. 2' Fluoro RNA A 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB201-IN004
    TVA inclus
    199,92 RON
  98. AP-dC 1000 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB190-IN100
    TVA inclus
    1.428,00 RON
  99. AP-dC 200 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB190-IN020
    TVA inclus
    1.128,12 RON
  100. AP-dC 40 nmol scale, 1 modification, Cod Catalog Eurogentec: MD-NB190-IN004
    TVA inclus
    721,14 RON
Setați descendent

Articolele 1-100 din 710

Pagină
pe pagină
Modifications
  1. Conjugates
  2. Degenerate bases
  3. Fluorescent dyes
  4. Modifiers
  5. Non natural bases
  6. Phosphates
  7. Quenchers
  8. Spacers
  9. Biotins
  10. NGS Modifications
  11. Purifications