PNA* (Peptide Nucleic Acid) an artificially created DNA analogue was invented by Drs. Nielsen, Egholm, Berg, and Buchardt in 1991. The uncharged backbone of PNA suppresses the charge repulsion between PNA and the DNA/RNA strand.

In comparison with DNA Oligonucleotides, PNA oligomers demonstrate a higher sequence specificity, improved stability, reproducibility and lower background.

 

The backbone of PNAs (Peptide Nucleic Acid) is made of repeating N-(2-aminoethyl)-glycine units linked by peptide bonds. Despite this radical structural change, PNAs are capable of sequence-specific binding in a helix form to its complementary DNA or RNA sequence. Due to its superior binding affinity and chemical/biological stability, PNA has been widely applied in the field of biology.



PNA FISH Probe Details

  Quantity Sequence Specificity
C-rich Telomere Probe 5 nmol repeats of CCCTAA For both human and mouse
G-rich Telomere Probe 5 nmol repeats of TTAGGG For both human and mouse
Centromere Probes 5 nmol AAACTAGACAGAAGCATT For both human and mouse
Centromere Protein B Probes 5 nmol ATTCGTTGGAAACGGGA Human specific



Centromere probe

Thanks to the high sequence specifity and improved stability of PNA, chromosome aberrations in the centromere such as trisomy, polysomy and break can be clearly detected by using PNA FISH probes.

Red fluorescent probe Green fluorescent probe

 

Telomere probe

In principle, fluorescence in situ hybridisation (FISH) should be able to provide information on telomere length of individual chromosomes. However the efficiency of DNA/RNA oligonucleotide hybridizations for telomeric repeats investigation is not sufficient. In comparison the use of PNA stabilizes PNA-DNA/RNA duplexes. When used with PNA probes, PNA-DNA hybridization is much faster within a couple of hours and results in significantly reduced background noise due to the high signal to background noise ratio.

Telomere C probe

Red fluorescent probe Green fluorescent probe

Telomere G probe

Red fluorescent probe Green fluorescent probe

 

Centromeres and telomeres co-staining

 

Telomere and centromere co-staining Telomere and centromere co-staining Telomere and centromere co-staining
Blood lymphocytes. Centromeres and telomeres were stained with FITC-labeled PNA probe and Cy-3-labeled PNA probe, respectively. Images supplied courtesy of Radhia M’kacher, Cell Environment Genetic Toxicology section 75020 Paris, France

 

Protocol of PNA Telomere probe for FISH 

(Download the PDF version)

PREPARATION

HYBRIDIZATION and WASHES

References

 

 

Please Find here below the PNA FISH Probe from PANAGENE.

PNA FISH - Telomere and Centromere Probes

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