C3026J, SHuffle T7 Competent E. coli - 12 transformation rxns

Chemically competent E. coli K12 cells engineered to form proteins containing disulfide bonds in the cytoplasm. Suitable for T7 promoter driven protein expression.

Highlights

• Engineered E. coli K12 to promote disulfide bond formation in the cytoplasm
• Constitutively expresses a chromosomal copy of the disufide bond isomerase DsbC
• DsbC promotes the correction of mis-oxidized proteins into their correct form
• The cytoplasmic DsbC is also a chaperone that can assist in the folding of proteins that do not require disulfide bonds
• Expresses a chromosomal copy of T7 RNAP
• Tight control of expression by lacI^q allows potentially toxic genes to be cloned
• Resistance to phage T1 (fhuA2), Nit, Str, Spec

Transformation Efficiency

1 x 10⁶ cfu/µg pUC19 DNA

Genotype

F´ lac, pro, lacI^q / Δ(ara-leu)7697 araD139 fhuA2 lacZ::T7 gene1 Δ(phoA)PvuII phoR ahpC* galE (or U) galK λatt::pNEB3-r1-cDsbC (Spec^R, lacI^q) ΔtrxB rpsL150(Str^R) Δgor Δ(malF)3