C3020K, NEB 10-beta Electrocompetent E. coli - 6 tubes

NEB 10-beta electrocompetent E. coli cells are optimized for high efficiency transformation by electroporation. These cells are ideal for DNA library constructions and all cloning purposes.

Highlights

• DH10B™ derivative
• Accomodation of large plasmids including BAC and cosmid constructs
• Efficient transformation of methylated DNA derived from eukaryotic sources and unmethylated DNA derived from PCR, cDNA and other sources
• Activity of nonspecific endonuclease I eliminated for highest quality plasmid preparations (endA1)
• Suitable for blue/white screening by α-complementation of the β-galactosidase gene (Φ80ΔlacZM15)
• Reduced recombination of cloned DNA (recA)
• Resistance to phage T1 (fhuA)
• K-12 Strain
• Free of animal products

Transformation Efficiency

> 2 x 10¹⁰ cfu/μg pUC19

Genotype

Δ(ara-leu) 7697 araD139  fhuA ΔlacX74 galK16 galE15 e14-  Φ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL (Str^R) rph spoT1 Δ(mrr-hsdRMS-mcrBC)