C3028J, SHuffle Express Competent E. coli - 12 transformation rxns

Chemically competent E. coli B cells engineered to form proteins containing disulfide bonds  in the cytoplasm.

Highlights

• Engineered E. coli B strain to promote disulfide bond formation in the cytoplasm
• Constitutively expresses a chromosomal copy of the disulfide bond isomerase DsbC
• DsbC promotes the correction of mis-oxidized proteins into their correct form (1,3)
• The cytoplasmic DsbC is also a chaperone that can assist in the folding of proteins that do not require disulfide bonds (4)
• Enhanced BL21 derivative
• Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
• Deficient in proteases Lon and OmpT
• Resistance to phage T1 (fhuA2), Nit, Spec, Str*
  *Resistance to low levels of streptomycin may be observed.

Transformation Efficiency

1 x 10⁷ cfu/µg pUC19 DNA

Genotype

fhuA2 [lon] ompT ahpC gal λatt::pNEB3-r1-cDsbC (Spec^R, lacI^q) ΔtrxB sulA11 R(mcr-73::miniTn10--Tet^S)2 [dcm] R(zgb-210::Tn10 --Tet^S) endA1 Δgor ∆(mcrC-mrr)114::IS10