C3013I, T7 Express lysY/lq Competent E. coli (High Efficiency) - 24 transformation reactions (6 tubes)

Chemically competent E. coli cells suitable for high efficiency transformation and protein expression.

Highlights

• Enhanced BL21 derivative for T7 expression
• T7 RNA Polymerase in the lac operon - no λ prophage
• Tight control of expression by lacl^q allows potentially toxic genes to be cloned
• Control of T7 RNA Polymerase by lysozyme allows toxic genes to be expressed
• Lys Y is a variant of T7 lysozyme lacking any amidase activity, thus cells are less susceptible to lysis during induction
• Maintenance of lysozyme/lacI^q plasmid does not require antibiotic selection
• Deficient in proteases Lon and OmpT
• Resistant to phage T1 (fhuA2)
• Does not restrict methylated DNA (McrA^-, McrBC^-, EcoBr^-m^-, Mrr^-)
• B Strain
• Free of animal products

Transformation Efficiency

0.6-1 x 10⁹ cfu/μg pUC19 DNA

Genotype

MiniF lysY lacI^q(Cam^R) / fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--Tet^S)2 [dcm] R(zgb-210::Tn10--Tet^S) endA1 Δ(mcrC-mrr) 114::IS10