C3019H, NEB 10-beta Competent E. coli (High Efficiency) - 20 transformation reactions

1.727,88 RON

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

SKU

NEB_C3019H

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

Highlights

DH10B™ derivative
Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Resistance to phage T1 (fhuA)
Suitable for blue/white screening without IPTG by a-complementation of the b-galactosidase gene
Reduced recombination of cloned DNA (recA1)
Clone large plasmids and BACs
Free of animal products
K12 Strain

Transformation Efficiency

For C3019I and C3019H: 1–3 x 10⁹ cfu/µg pUC19 DNA
For C3019P: 1–3 x 10⁸ cfu/µg pUC19 DNA

Genotype

Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (Str^R) rph spoT1 Δ(mrr-hsdRMS-mcrBC)

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Price	1.452,00 RON (preturile sunt fara TVA)
Description	Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications. Highlights DH10B™ derivative Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)] Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations Resistance to phage T1 (fhuA) Suitable for blue/white screening without IPTG by a-complementation of the b-galactosidase gene Reduced recombination of cloned DNA (recA1) Clone large plasmids and BACs Free of animal products K12 Strain Transformation Efficiency For C3019I and C3019H: 1–3 x 10⁹ cfu/µg pUC19 DNA For C3019P: 1–3 x 10⁸ cfu/µg pUC19 DNA Genotype Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (Str^R) rph spoT1 Δ(mrr-hsdRMS-mcrBC)