C3013I,  T7 Express lysY/lq Competent E. coli (High Efficiency)  - 24 transformation reactions (6 tubes)

C3013I, T7 Express lysY/lq Competent E. coli (High Efficiency) - 24 transformation reactions (6 tubes)

C3019I,  NEB 10-beta Competent E. coli (High Efficiency) - 24 transformation reactions (6 tubes)

C3019I, NEB 10-beta Competent E. coli (High Efficiency) - 24 transformation reactions (6 tubes)

C3019H, NEB 10-beta Competent E. coli (High Efficiency) - 20 transformation reactions

1.727,88 RON

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

SKU
NEB_C3019H

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

Highlights

  • DH10B™ derivative
  • Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
  • Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
  • Resistance to phage T1 (fhuA)
  • Suitable for blue/white screening without IPTG by a-complementation of the b-galactosidase gene
  • Reduced recombination of cloned DNA (recA1)
  • Clone large plasmids and BACs
  • Free of animal products
  • K12 Strain

Transformation Efficiency

For C3019I and C3019H:  1–3 x 109 cfu/µg pUC19 DNA
For C3019P: 1–3 x 108 cfu/µg pUC19 DNA 

Genotype

Δ(ara-leu) 7697 araD139  fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)

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Price 1.452,00 RON (preturile sunt fara TVA)
Description

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

Highlights

  • DH10B™ derivative
  • Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
  • Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
  • Resistance to phage T1 (fhuA)
  • Suitable for blue/white screening without IPTG by a-complementation of the b-galactosidase gene
  • Reduced recombination of cloned DNA (recA1)
  • Clone large plasmids and BACs
  • Free of animal products
  • K12 Strain

Transformation Efficiency

For C3019I and C3019H:  1–3 x 109 cfu/µg pUC19 DNA
For C3019P: 1–3 x 108 cfu/µg pUC19 DNA 

Genotype

Δ(ara-leu) 7697 araD139  fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)