C3010I, T7 Express lysY Competent E. coli (High Efficiency) - 24 transformation reactions (6 tubes)

Enhanced BL21 derivative, chemically competent E. coli cells suitable for high efficiency transformation and protein expression.

Highlights

• Free of animal products
• Control of T7 RNA Polymerase by lysozyme allows potentially toxic genes to be expressed
• LysY is a variant of T7 lysozyme lacking amidase activity, thus cells are less susceptible to lysis during induction
• Maintenance of lysozyme plasmid does not require antibiotic selection
• Enhanced BL21 derivative
• Does not restrict methylated DNA (McrA^-, McrBC^-, EcoBr^-m^-, Mrr^-)
• Deficient in proteases Lon and OmpT
• Lysozyme for expression control
• T7 RNA Polymerase in the lac operon - no λ prophage
• No Cam requirement
• Fast growth from colonies
• Lysozyme on miniF enhances stability
• Resistant to phage T1 (fhuA2) Cam, Nit
• B Strain

Transformation Efficiency

0.6-1 x 10⁹ cfu/μg pUC19 DNA

Genotype

MiniF lysY (Cam^R) / fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--Tet^S)2 [dcm] R(zgb-210::Tn10--Tet^S) endA1 ∆(mcrC-mrr)114::IS10