Takyon® qPCR Kits for SYBR® assays containing ROX passive reference are available in different MasterMixes formats from 1.5 ml to 10 x 7.5 ml. Takyon® qPCR Kits deliver FAST, SENSITIVE and ACCURATE results in FAST & regular conditions
For carry-over prevention, combine any dTTP MasterMix with the new dUTP/UNG additive for 150 rxns: RT-UTPUNG-005, for 750 rxns: RT-UTPUNG-020
To convert any dTTP 2X Mastermix into a One-step RT-qPCR Kit, please use the Takyon® One-step kit converter for 150 rxns: UF-RTAD-D0101, for 750 rxns: UF-RTAD-D0701
Avoid repeated freeze-thaw cycles.
Kits components |
Sample and 150 reactions kit contain: One tube (1.5 mL) of 2X MasterMix (blue cap - amber tube) containing Takyon® DNA polymerase, MgCl2 (2.5mM final concentration), SYBR Green®, dNTPs, ROX Passive reference, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 750 reactions kit contains each: One bottle (7.5 mL) of 2X MasterMix (blue cap - amber vial) containing Takyon® DNA polymerase, MgCl2 (2.5mM final concentration), SYBR Green®, dNTPs, ROX Passive reference, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) |
Properties |
|
Format |
2X MM |
Color |
Blue |
Quantity & Purity |
|
Concentration |
2 X |
Storage & stability |
|
Form |
In solution |
Storage Conditions |
For long term storage the Takyon® Rox SYBR® MasterMix dTTP blue should be stored at a temperature between -15°C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for 12 months. For short term storage the Takyon® Rox SYBR® MasterMix dTTP blue can be stored at 4 °C for 6 months. Product must be stored in the dark. |
Activity |
|
qPCR compatible thermocyclers |
ABI - Prism® SDS 5700 ABI - Prism® SDS 7000 ABI - Prism® SDS 7300 ABI - Prism® SDS 7700 ABI - Prism® SDS 7900/7900 HT ABI - StepOnePlus™ ABI - StepOne™ |
Application |
Fluorimetric |
Template type |
cDNA/DNA |
Volume activity |
20 µL |
Detection Method |
SYBR |
Usage |
Research use |
Price | 569,16 RON (preturile sunt fara TVA) | ||||||||||||||||||||||||||||||||
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Description |
Takyon® qPCR Kits for SYBR® assays containing ROX passive reference are available in different MasterMixes formats from 1.5 ml to 10 x 7.5 ml. Takyon® qPCR Kits deliver FAST, SENSITIVE and ACCURATE results in FAST & regular conditions For carry-over prevention, combine any dTTP MasterMix with the new dUTP/UNG additive for 150 rxns: RT-UTPUNG-005, for 750 rxns: RT-UTPUNG-020 To convert any dTTP 2X Mastermix into a One-step RT-qPCR Kit, please use the Takyon® One-step kit converter for 150 rxns: UF-RTAD-D0101, for 750 rxns: UF-RTAD-D0701 Avoid repeated freeze-thaw cycles.
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