UF-NP5X-RT0501, Takyon™ One-Step No Rox Probe 5X MasterMix dTTP, 1250 rxn (20 µl)

UF-NP5X-RT0501, Takyon™ One-Step No Rox Probe 5X MasterMix dTTP, 1250 rxn (20 µl)

UF-NSCT-B0201, Takyon No Rox SYBR Core Kit blue dTTP, 1250 rxn

3.313,67 RON
UF-NSCT-B0201, Takyon No Rox SYBR Core Kit blue dTTP, 1250 rxn
SKU
EUR_UF-NSCT-B0201

Takyon® Core Kits for qPCR SYBR assays containing no ROX passive reference are available in 1250 (20 µL) RXNs format. Takyon® Kits deliver FAST, SENSITIVE and ACCURATE results in FAST & Regular Cycling conditions. Core kits contain components in separated vials to allow maximum flexibility in assay formulation as the concentration of the different components can be optimized to get the best possible results.

For carry-over prevention, use dUTP/UNG additive included in the kits.

Avoid repeated freeze-thaw cycles.

Packaging

Kits components

Each kit contains: 2 Tubes (1.5 mL) of 10x Buffer (brown cap) containing KCl and Tris-HCl, Stabilizers, Inert Blue dye 2 Tubes (1.5 mL) of 50 mM MgCl2 (clear cap; optional use) 1 Tube (1.25 mL) of 5 mM dNTPs (green cap) containing a blend of dATP, dCTP, dGTP and dTTP 1 Tube (125 µL) of Takyon® enzyme (5 U/µL) (yellow cap) 1 Tube (1 mL) of DMSO 1 Tube (330 µL) of dUTP and Uracyl N-glycosylase blend for carryover prevention 1 tube of SYBR® Green I (amber tube)

Properties

Format

Core Kit

Color

Blue

Quantity & Purity

Concentration

Separated components

Storage & stability

Form

In solution

Storage Conditions

For long term storage the Takyon® No Rox SYBR® Core Kit dTTP blue should be stored at a temperature between -15 °C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for 24 months. For short term storage the Takyon® No Rox SYBR® Core Kit dTTP blue can be stored at 4°C for 6 months. The SYBR® Green I is light sensitive and should be kept away from light as much as possible.

Activity

qPCR compatible thermocyclers

           

Analytik jena - qTower

Analytik jena - qTOWER³

Analytik jena - qTOWERiris

Bio Molecular Systems - Mic

BioRad - CFX Duet

BioRad - CFX384®

BioRad - CFX96®

BioRad - DNA Engine Opticon™ 1

BioRad - DNA Engine Opticon™ 2

BioRad - iCycler™

BioRad - My iQ™

BioRad- Chromo 4™

Cepheid - Smartcycler™ 1

Cepheid - Smartcycler™ 2

Esco - Swift™ Spectrum 96

PCRmax - Eco 48

Qiagen - QIAquant 384

Qiagen - QIAquant 96

Qiagen/Corbett - Rotor-Gene™ 2000

Qiagen/Corbett - Rotor-Gene™ 3000

Qiagen/Corbett - Rotor-Gene™ 6000

Qiagen/Corbett - Rotor-Gene™ Q

Roche - LightCycler® 480

Roche - LightCycler® 96

Roche - LightCycler® Nano

Roche - LightCycler® PRO

Stratagene/Agilent - AriaMX

Stratagene/Agilent - Mx3000P™ (without ROX normalisation)

Stratagene/Agilent - Mx3005P™ (without ROX normalisation)

Stratagene/Agilent - Mx4000™ (without ROX normalisation)

Techne - Quantica™

Application

Fluorimetric

Template type

cDNA/DNA

Volume activity

20 µL

Detection Method

SYBR

Usage

Research use

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Description

Takyon® Core Kits for qPCR SYBR assays containing no ROX passive reference are available in 1250 (20 µL) RXNs format. Takyon® Kits deliver FAST, SENSITIVE and ACCURATE results in FAST & Regular Cycling conditions. Core kits contain components in separated vials to allow maximum flexibility in assay formulation as the concentration of the different components can be optimized to get the best possible results.

For carry-over prevention, use dUTP/UNG additive included in the kits.

Avoid repeated freeze-thaw cycles.

Packaging

Kits components

Each kit contains: 2 Tubes (1.5 mL) of 10x Buffer (brown cap) containing KCl and Tris-HCl, Stabilizers, Inert Blue dye 2 Tubes (1.5 mL) of 50 mM MgCl2 (clear cap; optional use) 1 Tube (1.25 mL) of 5 mM dNTPs (green cap) containing a blend of dATP, dCTP, dGTP and dTTP 1 Tube (125 µL) of Takyon® enzyme (5 U/µL) (yellow cap) 1 Tube (1 mL) of DMSO 1 Tube (330 µL) of dUTP and Uracyl N-glycosylase blend for carryover prevention 1 tube of SYBR® Green I (amber tube)

Properties

Format

Core Kit

Color

Blue

Quantity & Purity

Concentration

Separated components

Storage & stability

Form

In solution

Storage Conditions

For long term storage the Takyon® No Rox SYBR® Core Kit dTTP blue should be stored at a temperature between -15 °C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for 24 months. For short term storage the Takyon® No Rox SYBR® Core Kit dTTP blue can be stored at 4°C for 6 months. The SYBR® Green I is light sensitive and should be kept away from light as much as possible.

Activity

qPCR compatible thermocyclers

           

Analytik jena - qTower

Analytik jena - qTOWER³

Analytik jena - qTOWERiris

Bio Molecular Systems - Mic

BioRad - CFX Duet

BioRad - CFX384®

BioRad - CFX96®

BioRad - DNA Engine Opticon™ 1

BioRad - DNA Engine Opticon™ 2

BioRad - iCycler™

BioRad - My iQ™

BioRad- Chromo 4™

Cepheid - Smartcycler™ 1

Cepheid - Smartcycler™ 2

Esco - Swift™ Spectrum 96

PCRmax - Eco 48

Qiagen - QIAquant 384

Qiagen - QIAquant 96

Qiagen/Corbett - Rotor-Gene™ 2000

Qiagen/Corbett - Rotor-Gene™ 3000

Qiagen/Corbett - Rotor-Gene™ 6000

Qiagen/Corbett - Rotor-Gene™ Q

Roche - LightCycler® 480

Roche - LightCycler® 96

Roche - LightCycler® Nano

Roche - LightCycler® PRO

Stratagene/Agilent - AriaMX

Stratagene/Agilent - Mx3000P™ (without ROX normalisation)

Stratagene/Agilent - Mx3005P™ (without ROX normalisation)

Stratagene/Agilent - Mx4000™ (without ROX normalisation)

Techne - Quantica™

Application

Fluorimetric

Template type

cDNA/DNA

Volume activity

20 µL

Detection Method

SYBR

Usage

Research use