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UF-NP5X-RT0501, Takyon™ One-Step No Rox Probe 5X MasterMix dTTP, 1250 rxn (20 µl)
9.300,14 RON
UF-NP5X-RT0501, Takyon™ One-Step No Rox Probe 5X MasterMix dTTP, 1250 rxn (20 µl)
SKU
EUR_UF-NP5X-RT0501
Takyon™ One-Step 5X qRT-PCR Kits for Probe assays containing no ROX passive reference are available in different MasterMix formats from 0.6 ml to 10 x 5 ml. Takyon™ 5X qRT-PCR Kits deliver FAST, SENSITIVE and ACCURATE results in fast & regular cycling conditions.
These kits are perfectly suited for gene expression studies and demonstrate exceptional performance on viral templates thanks to the newly developped EuroscriptII RT
Moreover the new additive allows a significant improvement of the performance on some difficult virus types as well as on FFPE samples.
Avoid repeated freeze-thaw cycles.
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Kits components |
Sample and 150 reactions kit contain: One tube (0.6 mL) of 5x MasterMix (black cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 30 µL of Euroscript II RT (50 u/µL) / RNAse inhibitor(20u/µL) (white cap) 30 µL of Additive (blue cap) that improve results on some viral and FFPE samples 1250 reactions kit contains each: Five tubes (1 mL) of 5x MasterMix (black cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 250 µL of Euroscript II RT (50 u/µL) / RNAse inhibitor(20u/µL) (white cap) 250 µL of Additive (blue cap) that improve results on some viral and FFPE samples |
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Properties |
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Format |
5X MM |
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Color |
Clear |
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Quantity & Purity |
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Concentration |
5 X |
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Storage & stability |
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Form |
In solution |
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Storage Conditions |
5X MasterMix components of the Takyon™ One-Step No Rox 5X Probe MasterMix dTTP should be stored between -15 °C and -25°C in a constant temperature freezer. When stored under these conditions, this component is stable for 24 months. For short term storage the Takyon™ One-Step No Rox Probe 5X MasterMix dTTP can be stored at 4 °C for 6 months. - Euroscript II RT & additive should be stored at a temperature between -65°C and -75°C in a constant temperature freezer. When stored under these conditions, the reagents are stable for 1 year. For short term storage the Euroscript II RT and additive can be stored between -15°C and -25°C for 1 month. |
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Activity |
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qPCR compatible thermocyclers |
Analytik jena - qTower Analytik jena - qTOWER³ Analytik jena - qTOWERiris Bio Molecular Systems - Mic BioRad - CFX Duet BioRad - CFX384® BioRad - CFX96® BioRad - DNA Engine Opticon™ 1 BioRad - DNA Engine Opticon™ 2 BioRad - iCycler™ BioRad - My iQ™ BioRad- Chromo 4™ Cepheid - Smartcycler™ 1 Cepheid - Smartcycler™ 2 Esco - Swift™ Spectrum 96 PCRmax - Eco 48 Qiagen - QIAquant 384 Qiagen - QIAquant 96 Qiagen/Corbett - Rotor-Gene™ 2000 Qiagen/Corbett - Rotor-Gene™ 3000 Qiagen/Corbett - Rotor-Gene™ 6000 Qiagen/Corbett - Rotor-Gene™ Q Roche - LightCycler® 480 Roche - LightCycler® 96 Roche - LightCycler® Nano Roche - LightCycler® PRO Stratagene/Agilent - AriaMX Stratagene/Agilent - Mx3000P™ (without ROX normalisation) Stratagene/Agilent - Mx3005P™ (without ROX normalisation) Stratagene/Agilent - Mx4000™ (without ROX normalisation) Techne - Quantica™ |
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Application |
Fluorimetric |
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Template type |
cDNA/DNA |
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Volume activity |
20 µL |
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Detection Method |
Probe |
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Usage |
Research use |
| Price | 7.815,24 RON (preturile sunt fara TVA) | ||||||||||||||||||||||||||||||||
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| Description |
Takyon™ One-Step 5X qRT-PCR Kits for Probe assays containing no ROX passive reference are available in different MasterMix formats from 0.6 ml to 10 x 5 ml. Takyon™ 5X qRT-PCR Kits deliver FAST, SENSITIVE and ACCURATE results in fast & regular cycling conditions. These kits are perfectly suited for gene expression studies and demonstrate exceptional performance on viral templates thanks to the newly developped EuroscriptII RT Moreover the new additive allows a significant improvement of the performance on some difficult virus types as well as on FFPE samples. Avoid repeated freeze-thaw cycles.
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