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T3005, Mix & Go! Competent Cells-JM109 (96 x 50 ul)
4.153,10 RON
The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/ µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
SKU
ZR_T3005
HIGHLIGHTS
- Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
- High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
- Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Additional Info | Partly restriction-deficient; good strain for cloning repetitive DNA (recA-). Suppresses many amber mutations when glutamine is available but not the S100 or S7 mutation of λ, e.g.,λgt11. Can be used for M13 cloning/sequencing and blue/white screening. |
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| Genotype | F`[traD36 proA+B+ laclq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1 |
| Processing Time | 20 Seconds |
| Product Storage | -70°C to -80°C |
| Transformation Efficiency | 108 - 109 transformants per µg of plasmid DNA |
| Supplemental Info |
| Price | 3.490,00 RON (preturile sunt fara TVA) | ||||||||||||
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| Description |
HIGHLIGHTS
DESCRIPTIONTECHNICAL SPECIFICATIONS
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| Manufacturer | Zymo Research |
