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T3002, Mix & Go! E.coli Transformation Buffer Set (up to 60 ml)

T3002, Mix & Go! E.coli Transformation Buffer Set (up to 60 ml)

T3005, Mix & Go! Competent Cells-JM109 (96 x 50 ul)

T3005, Mix & Go! Competent Cells-JM109 (96 x 50 ul)

T3003, Mix & Go! Competent Cells-JM109 (10 x 100 ul)

1.088,85 RON
The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/ µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
SKU
ZR_T3003
Adaugati pentru comparare
HIGHLIGHTS
  • Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
  • High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
  • Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.
DESCRIPTION

The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/ µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.

Learn More

TECHNICAL SPECIFICATIONS
Additional Info Partly restriction-deficient; good strain for cloning repetitive DNA (recA-). Suppresses many amber mutations when glutamine is available but not the S100 or S7 mutation of λ, e.g.,λgt11. Can be used for M13 cloning/sequencing and blue/white screening.
Genotype F`[traD36 proA+B+ laclq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1
Processing Time 20 Seconds
Product Storage -70°C to -80°C
Transformation Efficiency 108 - 109 transformants per µg of plasmid DNA
Supplemental Info
Mai multe informatii
Price 915,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS
  • Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
  • High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
  • Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.
DESCRIPTION

The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/ µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.

Learn More

TECHNICAL SPECIFICATIONS
Additional Info Partly restriction-deficient; good strain for cloning repetitive DNA (recA-). Suppresses many amber mutations when glutamine is available but not the S100 or S7 mutation of λ, e.g.,λgt11. Can be used for M13 cloning/sequencing and blue/white screening.
Genotype F`[traD36 proA+B+ laclq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1
Processing Time 20 Seconds
Product Storage -70°C to -80°C
Transformation Efficiency 108 - 109 transformants per µg of plasmid DNA
Supplemental Info
Manufacturer Zymo Research
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