ELK9742-96T, Cattle NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit, 96T

ELK9742-96T, Cattle NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit, 96T

ELK9743-96T, Cattle SPD(Surfactant Associated Protein D) ELISA Kit, 96T

ELK9743-96T, Cattle SPD(Surfactant Associated Protein D) ELISA Kit, 96T

ELK9743-48T, Cattle SPD(Surfactant Associated Protein D) ELISA Kit, 48T

1.814,75 RON

Cattle SPD(Surfactant Associated Protein D) ELISA Kit

SKU
ELK9743-48T

Alternative Names: SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D

Species: Cattle

Assay Type: Sandwich

Sensitivity: 0.237 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Infection immunity;Pulmonology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle SPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle SPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle SPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle SPD in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D

Species: Cattle

Assay Type: Sandwich

Sensitivity: 0.237 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Infection immunity;Pulmonology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle SPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle SPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle SPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle SPD in the samples is then determined by comparing the OD of the samples to the standard curve.