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ELK9742-96T, Cattle NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit, 96T
2.963,10 RON
Cattle NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit
SKU
ELK9742-96T
Alternative Names: NT-Pro-ANP; N-ANP
Species: Cattle
Assay Type: Sandwich
Sensitivity: 0.251 ng/mL
Standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: NT-Pro-ANP; N-ANP Species: Cattle Assay Type: Sandwich Sensitivity: 0.251 ng/mL Standard: 40 ng/mL Detection range: 0.63-40 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids. Assay length: 3.5h Research Area: Endocrinology;Cardiovascular biology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve. |
