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ELK9598-48T, Human C3d(ComplementFragment 3d) ELISA Kit, 48T
2.142,00 RON
Human C3d(ComplementFragment 3d) ELISA Kit
SKU
ELK9598-48T
Alternative Names: C3d
Species: Human
Assay Type: Sandwich
Sensitivity: 4.69 ng/mL
Standard: 500 ng/mL
Detection range: 7.81-500 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Immunology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3d. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3d. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3d, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3d in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: C3d Species: Human Assay Type: Sandwich Sensitivity: 4.69 ng/mL Standard: 500 ng/mL Detection range: 7.81-500 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Immunology Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3d. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3d. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3d, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3d in the samples is then determined by comparing the OD of the samples to the standard curve. |
