ELK9597-96T, Human MR-ProADM(Mid-regional pro-adrenomedullin) ELISA Kit, 96T

Alternative Names: MR-ProADM

Species: Human

Assay Type: Sandwich

Sensitivity: 9.38 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MR-ProADM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MR-ProADM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MR-ProADM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MR-ProADM in the samples is then determined by comparing the OD of the samples to the standard curve.