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ELK8664-48T, Rat TBA(Total Bile Acid) ELISA Kit, 48T
2.142,00 RON
Rat TBA(Total Bile Acid) ELISA Kit
SKU
ELK8664-48T
Alternative Names: TBA; Total Bile Acid
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.91 ng/mL
Standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Hepatic function
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TBA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TBA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TBA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TBA in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: TBA; Total Bile Acid Species: Rat Assay Type: Sandwich Sensitivity: 0.91 ng/mL Standard: 200 ng/mL Detection range: 3.13-200 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Hepatic function Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TBA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TBA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TBA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TBA in the samples is then determined by comparing the OD of the samples to the standard curve. |
