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ELK8663-96T, Rat MAU(Microalbuminuria) ELISA Kit, 96T
2.963,10 RON
Rat MAU(Microalbuminuria) ELISA Kit
SKU
ELK8663-96T
Alternative Names: MAU; Microalbuminuria
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.48 µg/mL
Standard: 100 µg/mL
Detection range: 1.57-100 µg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Diabetes
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAU in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: MAU; Microalbuminuria Species: Rat Assay Type: Sandwich Sensitivity: 0.48 µg/mL Standard: 100 µg/mL Detection range: 1.57-100 µg/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Diabetes Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAU in the samples is then determined by comparing the OD of the samples to the standard curve. |
