ELK8534-48T, Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit, 48T

Alternative Names: C-RP; PTX1; Pentraxin-Related; Pentraxin 1; hs-CRP

Species: Human

Assay Type: Sandwich

Sensitivity: 0.51 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.