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ELK8533-96T, Human 8-OHdG(DNA Damage) ELISA Kit, 96T
2.475,20 RON
Human 8-OHdG(DNA Damage) ELISA Kit
SKU
ELK8533-96T
Alternative Names: 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 8-Hydroxydeoxyguanosine
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 26.29 pg/mL
Standard: 6000 pg/mL
Detection range: 93.75-6000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 8-Hydroxydeoxyguanosine Species: Human Assay Type: Competitive Inhibition Sensitivity: 26.29 pg/mL Standard: 6000 pg/mL Detection range: 93.75-6000 pg/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve. |
