ELK8420-48T, Human Total cholesterol (TC) ELISA Kit, 48T

Alternative Names: TC

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.63 µg/mL

Standard: 500 µg/mL

Detection range: 7.82-500 µg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TC in the samples is then determined by comparing the OD of the samples to the standard curve.