Human SA(Sialic Acid) ELISA Kit
Alternative Names: SA
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 3.95 µg/mL
Standard: 500 µg/mL
Detection range: 7.82-500 µg/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 2.5h
Research Area: Signal transduction;Tumor immunity;Hepatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SA in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.490,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: SA Species: Human Assay Type: Competitive Inhibition Sensitivity: 3.95 µg/mL Standard: 500 µg/mL Detection range: 7.82-500 µg/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids. Assay length: 2.5h Research Area: Signal transduction;Tumor immunity;Hepatology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SA in the samples is then determined by comparing the OD of the samples to the standard curve. |