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ELK8382-96T, Human Barbiturates ELISA Kit, 96T
2.963,10 RON
Human Barbiturates ELISA Kit
SKU
ELK8382-96T
Alternative Names: Barbituates
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 0.1 ng/mL
Standard: 25 ng/mL
Detection range: 0.4-25 ng/mL
Sample type: Plasma, Serum, Urine
Assay length: 2h
Research Area: Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Barbitues protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Barbitues. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Barbitues in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Barbituates Species: Human Assay Type: Competitive Inhibition Sensitivity: 0.1 ng/mL Standard: 25 ng/mL Detection range: 0.4-25 ng/mL Sample type: Plasma, Serum, Urine Assay length: 2h Research Area: Neuro science; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Barbitues protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Barbitues. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Barbitues in the samples is then determined by comparing the OD of the samples to the standard curve. |
