ELK8382-48T, Human Barbiturates ELISA Kit, 48T

Alternative Names: Barbituates

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.1 ng/mL

Standard: 25 ng/mL

Detection range: 0.4-25 ng/mL

Sample type: Plasma, Serum, Urine

Assay length: 2h

Research Area: Neuro science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Barbitues protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Barbitues. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Barbitues in the samples is then determined by comparing the OD of the samples to the standard curve.