DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit
Alternative Names: Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 4.25 ng/mL
Standard: 400 ng/mL
Detection range: 6.25-400 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;Rheumatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHEA-S protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHEA-S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.490,00 RON (preturile sunt fara TVA) |
---|---|
Description |
Alternative Names: Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate Species: General Assay Type: Competitive Inhibition Sensitivity: 4.25 ng/mL Standard: 400 ng/mL Detection range: 6.25-400 ng/mL Sample type: Serum, plasma and other biological fluids. Assay length: 2h Research Area: Endocrinology;Reproductive science;Hormone metabolism;Rheumatology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHEA-S protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHEA-S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve. |