ELK8369-96T, Mouse WNT2(Wingless Type MMTV Integration Site Family, Member 2) ELISA Kit, 96T

ELK8369-96T, Mouse WNT2(Wingless Type MMTV Integration Site Family, Member 2) ELISA Kit, 96T

ELK8370-96T, DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit, 96T

ELK8370-96T, DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit, 96T

ELK8370-48T, DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit, 48T

2.142,00 RON

DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit

SKU
ELK8370-48T

Alternative Names: Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 4.25 ng/mL

Standard: 400 ng/mL

Detection range: 6.25-400 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 2h

Research Area: Endocrinology;Reproductive science;Hormone metabolism;Rheumatology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHEA-S protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHEA-S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 4.25 ng/mL

Standard: 400 ng/mL

Detection range: 6.25-400 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 2h

Research Area: Endocrinology;Reproductive science;Hormone metabolism;Rheumatology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHEA-S protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHEA-S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.