ELK8222-48T, Horse COL1(Collagen Type I) ELISA Kit, 48T

Alternative Names: COL1; COLI

Species: Horse

Assay Type: Competitive Inhibition

Sensitivity: 7.15 ng/mL

Standard: 1500 ng/mL

Detection range: 23.44-1500 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Developmental science;Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse COL1 in the samples is then determined by comparing the OD of the samples to the standard curve.