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ELK8221-96T, Horse COL3(Collagen Type III) ELISA Kit, 96T
3.736,60 RON
Horse COL3(Collagen Type III) ELISA Kit
SKU
ELK8221-96T
Alternative Names: Collagen Type 3; Col 3; Collagen Type III; Col III
Species: Horse
Assay Type: Competitive Inhibition
Sensitivity: 4.98 ng/mL
Standard: 1000 ng/mL
Detection range: 15.63-1000 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse COL3 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Collagen Type 3; Col 3; Collagen Type III; Col III Species: Horse Assay Type: Competitive Inhibition Sensitivity: 4.98 ng/mL Standard: 1000 ng/mL Detection range: 15.63-1000 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Cardiovascular biology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse COL3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
