ELK8037-96T, Human Anti-HPF4(Anti-Heparin/Platelet Factor 4 Antibody) ELISA Kit, 96T

ELK8037-96T, Human Anti-HPF4(Anti-Heparin/Platelet Factor 4 Antibody) ELISA Kit, 96T

ELK8038-96T, Sheep TNFa(Tumor Necrosis Factor Alpha) ELISA Kit, 96T

ELK8038-96T, Sheep TNFa(Tumor Necrosis Factor Alpha) ELISA Kit, 96T

ELK8038-48T, Sheep TNFa(Tumor Necrosis Factor Alpha) ELISA Kit, 48T

2.689,40 RON

Sheep TNFa(Tumor Necrosis Factor Alpha) ELISA Kit

SKU
ELK8038-48T

Alternative Names: DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

Species: Sheep

Assay Type: Sandwich

Sensitivity: 1.21 pg/mL

Standard: 200 pg/mL

Detection range: 3.13-200 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.

Mai multe informatii
Price 2.260,00 RON (preturile sunt fara TVA)
Description

Alternative Names: DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

Species: Sheep

Assay Type: Sandwich

Sensitivity: 1.21 pg/mL

Standard: 200 pg/mL

Detection range: 3.13-200 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.