ELK8037-48T, Human Anti-HPF4(Anti-Heparin/Platelet Factor 4 Antibody) ELISA Kit, 48T

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ELK8037-96T, Human Anti-HPF4(Anti-Heparin/Platelet Factor 4 Antibody) ELISA Kit, 96T

2.963,10 RON

Human Anti-HPF4(Anti-Heparin/Platelet Factor 4 Antibody) ELISA Kit

SKU
ELK8037-96T

Alternative Names: HPF4 Ab

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.59 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 2.5h

Research Area: Hematology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-HPF4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-HPF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-HPF4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: HPF4 Ab

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.59 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 2.5h

Research Area: Hematology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-HPF4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-HPF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-HPF4 in the samples is then determined by comparing the OD of the samples to the standard curve.