ELK7840-48T, 1,5-AG(1,5-Anhydroglucitol) ELISA Kit, 48T

Alternative Names: LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.6 µg/mL

Standard: 150 µg/mL

Detection range: 2.35-150 µg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve.