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ELK7839-96T, bHB(Beta-Hydroxybutyric Acid) ELISA Kit, 96T
2.475,20 RON
bHB(Beta-Hydroxybutyric Acid) ELISA Kit
SKU
ELK7839-96T
Alternative Names: OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.48 µg/mL
Standard: 100 µg/mL
Detection range: 1.57-100 µg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Signal transduction;Metabolic pathway;Endocrinology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with bHB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to bHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of bHB in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid Species: General Assay Type: Competitive Inhibition Sensitivity: 0.48 µg/mL Standard: 100 µg/mL Detection range: 1.57-100 µg/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Signal transduction;Metabolic pathway;Endocrinology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with bHB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to bHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of bHB in the samples is then determined by comparing the OD of the samples to the standard curve. |
