ELK7628-48T, Dog OC(Osteocalcin) ELISA Kit, 48T

Alternative Names: BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein

Species: Dog

Assay Type: Sandwich

Sensitivity: 0.249 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog OC in the samples is then determined by comparing the OD of the samples to the standard curve.