ELK7627-48T, Human MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit, 48T

ELK7627-48T, Human MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit, 48T

ELK7628-48T, Dog OC(Osteocalcin) ELISA Kit, 48T

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ELK7627-96T, Human MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit, 96T

2.963,10 RON

Human MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit

SKU
ELK7627-96T

Alternative Names: MOTS-c

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.99 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Developmental science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MOTS-c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: MOTS-c

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.99 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Developmental science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MOTS-c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve.