ELK3190-48T, Human NPTX2(Neuronal Pentraxin II) ELISA Kit, 48T

ELK3190-48T, Human NPTX2(Neuronal Pentraxin II) ELISA Kit, 48T

ELK3192-48T, Mouse SCT(Secretin) ELISA Kit, 48T

ELK3192-48T, Mouse SCT(Secretin) ELISA Kit, 48T

ELK3190-96T, Human NPTX2(Neuronal Pentraxin II) ELISA Kit, 96T

2.963,10 RON

Human NPTX2(Neuronal Pentraxin II) ELISA Kit

SKU
ELK3190-96T

Alternative Names: NARP2; NP-II; NP2; Apexin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.062 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NPTX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPTX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NPTX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPTX2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: NARP2; NP-II; NP2; Apexin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.062 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NPTX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPTX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NPTX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPTX2 in the samples is then determined by comparing the OD of the samples to the standard curve.