ELK0768-48T, Human HS(Heparan Sulfate) ELISA Kit, 48T

Alternative Names: HS; Heparan Sulfate

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 51.3 ng/mL

Standard: 8000 ng/mL

Detection range: 125-8000 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HS in the samples is then determined by comparing the OD of the samples to the standard curve.