Human KS(Keratan Sulfate) ELISA Kit
Alternative Names: KS; Keratan Sulfate
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 31.2 pg/mL
Standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KS in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.080,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: KS; Keratan Sulfate Species: Human Assay Type: Competitive Inhibition Sensitivity: 31.2 pg/mL Standard: 5000 pg/mL Detection range: 78.13-5000 pg/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KS in the samples is then determined by comparing the OD of the samples to the standard curve. |