ELK0767-48T, Human KS(Keratan Sulfate) ELISA Kit, 48T

ELK0767-48T, Human KS(Keratan Sulfate) ELISA Kit, 48T

ELK0768-48T, Human HS(Heparan Sulfate) ELISA Kit, 48T

ELK0768-48T, Human HS(Heparan Sulfate) ELISA Kit, 48T

ELK0767-96T, Human KS(Keratan Sulfate) ELISA Kit, 96T

2.475,20 RON

Human KS(Keratan Sulfate) ELISA Kit

SKU
ELK0767-96T

Alternative Names: KS; Keratan Sulfate

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 31.2 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KS in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: KS; Keratan Sulfate

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 31.2 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KS in the samples is then determined by comparing the OD of the samples to the standard curve.