ELK0726-48T, Human miRNA-210 ELISA Kit, 48T

Alternative Names: microRNA-210

Species: Human

Assay Type: Sandwich

Sensitivity: 12.23 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human miRNA-210. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human miRNA-210. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human miRNA-210, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human miRNA-210 in the samples is then determined by comparing the OD of the samples to the standard curve.