ELK0725-48T, Human SLC17A5(Sialin) ELISA Kit, 48T

ELK0725-48T, Human SLC17A5(Sialin) ELISA Kit, 48T

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ELK0725-96T, Human SLC17A5(Sialin) ELISA Kit, 96T

2.963,10 RON

Human SLC17A5(Sialin) ELISA Kit

SKU
ELK0725-96T

Alternative Names: H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter (AST); Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular H(+)/Aspartate-glutamate cotransporter

Species: Human

Assay Type: Sandwich

Sensitivity: 0.051 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SLC17A5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SLC17A5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SLC17A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SLC17A5 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter (AST); Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular H(+)/Aspartate-glutamate cotransporter

Species: Human

Assay Type: Sandwich

Sensitivity: 0.051 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SLC17A5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SLC17A5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SLC17A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SLC17A5 in the samples is then determined by comparing the OD of the samples to the standard curve.