D5220, EZ Nucleosomal DNA Prep Kit (20 Preps), [Includes Room Temp item D5220 x 1 and Dry Ice Item D5220-1 x 1]
1.017,45 RON
The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome-associated DNA. The kit includes reagents/procedures for the following: cell nuclei isolation, intact nuclei enzymatic digestion, and nucleosomal DNA purification. This kit includes two different enzymes for nucleosomal DNA preparation: Atlantis dsDNase and Micrococcal Nuclease. Enzymatic digestion yields very homogeneous populations of core nucleosomes and purification of the nucleosome-associated DNA is performed using Zymo Research’s proven spin column technology. The result is pure nucleosomal DNA ready for analysis in less than 45 minutes!
For the isolation of nucleosome-associated DNA from mammalian and yeast cells.
Ideal for use in nucleosome mapping studies.
DESCRIPTION
The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome-associated DNA. The kit includes reagents/procedures for the following: cell nuclei isolation, intact nuclei enzymatic digestion, and nucleosomal DNA purification. This kit includes two different enzymes for nucleosomal DNA preparation: Atlantis dsDNase and Micrococcal Nuclease. Enzymatic digestion yields very homogeneous populations of core nucleosomes and purification of the nucleosome-associated DNA is performed using Zymo Research’s proven spin column technology. The result is pure nucleosomal DNA ready for analysis in less than 45 minutes!
TECHNICAL SPECIFICATIONS
Applicable For
All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Processing Volume
≤106 cells
Purity
Typical A260/A280 ≥ 1.8
Sample Source
Nucleosome associated DNA isolation and purification from mammalian and yeast cells.
Sample Storage
Eluted DNA should be stored at ≤ -20°C.
Yield
Up to 25 µg total DNA can be eluted into ≥ 25 µl. For DNA 75 bp - 10 kb the recovery is 70-90%. For DNA 11 kb - 23 kb the recovery is 50-70%.
For the isolation of nucleosome-associated DNA from mammalian and yeast cells.
Ideal for use in nucleosome mapping studies.
DESCRIPTION
The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome-associated DNA. The kit includes reagents/procedures for the following: cell nuclei isolation, intact nuclei enzymatic digestion, and nucleosomal DNA purification. This kit includes two different enzymes for nucleosomal DNA preparation: Atlantis dsDNase and Micrococcal Nuclease. Enzymatic digestion yields very homogeneous populations of core nucleosomes and purification of the nucleosome-associated DNA is performed using Zymo Research’s proven spin column technology. The result is pure nucleosomal DNA ready for analysis in less than 45 minutes!
TECHNICAL SPECIFICATIONS
Applicable For
All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Processing Volume
≤106 cells
Purity
Typical A260/A280 ≥ 1.8
Sample Source
Nucleosome associated DNA isolation and purification from mammalian and yeast cells.
Sample Storage
Eluted DNA should be stored at ≤ -20°C.
Yield
Up to 25 µg total DNA can be eluted into ≥ 25 µl. For DNA 75 bp - 10 kb the recovery is 70-90%. For DNA 11 kb - 23 kb the recovery is 50-70%.