D5220, EZ Nucleosomal DNA Prep Kit (20 Preps), [Includes Room Temp item D5220 x 1 and Dry Ice Item D5220-1 x 1]
D5220-2, Nuclei Prep Buffer (50 ml)
D5220-1, Micrococcal Nuclease (10 U /100 ?l)
249,90 RON
Micrococcal Nuclease cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3′-phosphates.
Micrococcal Nuclease cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3′-phosphates.
TECHNICAL SPECIFICATIONS
Concentration
0.1 U/µl
Enzyme Commission Number
(E.C. 3.1.31.1)
Enzyme Inactivation
EDTA or EGTA in molar excess of CaCl2.
Storage
Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤ -70 °C.
Unit Definition
One unit (U) will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10,000 for the mixed nucleotides.
Micrococcal Nuclease cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3′-phosphates.
TECHNICAL SPECIFICATIONS
Concentration
0.1 U/µl
Enzyme Commission Number
(E.C. 3.1.31.1)
Enzyme Inactivation
EDTA or EGTA in molar excess of CaCl2.
Storage
Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤ -70 °C.
Unit Definition
One unit (U) will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10,000 for the mixed nucleotides.