Quickly and easily purify high quality DNA from PCR and other enzymatic reactions.
Elute in as little as 6 μl
Prevent buffer retention and salt carry-over with optimized column design
Save time with fast, user-friendly protocols
No need to monitor pH
Protocol modification allows for ssDNA purification, oligonucleotide purification, and purification of other small DNA fragments
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Responsibly-sourced and recyclable packaging
APPLICATIONS
PCR cleanup
DNA from PCR reactions can be purified after amplification to remove polymerases, primers, detergents, dNTPs, etc.
Enzymatic reaction cleanup
Restriction enzymes and modifying enzymes such as ligases, kinases, nucleases, phosphatases are efficiently removed, allowing for effective desalting and concentration of the DNA sample.
cDNA cleanup
DNA/RNA complexes can be purified post-reverse transcription/amplification to enable removal of the RT and polymerase as well as nucleotides.
Labeling cleanup
Unincorporated radiolabeled or fluorescently labeled nucleotides can be removed from the DNA substrate
Plasmid cleanup
Plasmid preps from unknown sources may contain inhibitors and unwanted contaminants. Purification and concentration can be easily achieved using this kit.
Oligonucleotide cleanup
ssDNA oligonucleotides (≥ 18 nt) and dsDNA fragments (≥ 15 bp) can be purified using the Oligonucleotide Cleanup Protocol.
Characteristics
DNA Sample Type:
DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations). ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol.
Binding Capacity:
up to 5 μg
DNA Size Range:
~50 bp to 25 kb DNA ≥ 15 bp to 25 kb (dsDNA) and DNA ≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol.
Typical Recovery:
DNA (50 bp to 10 kb): 70–90% DNA (11–23 kb): 50–70% ssDNA ≥ 18 nt and dsDNA ≥ 15 bp: 70-85%
Elution Volume:
≥ 6 μl
Purity:
A260/280 > 1.8 and A260/230 > 1.8
Protocol Time:
5 minutes of spin and incubation time
Compatible Downstream Applications:
ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing
Quickly and easily purify high quality DNA from PCR and other enzymatic reactions.
Elute in as little as 6 μl
Prevent buffer retention and salt carry-over with optimized column design
Save time with fast, user-friendly protocols
No need to monitor pH
Protocol modification allows for ssDNA purification, oligonucleotide purification, and purification of other small DNA fragments
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Responsibly-sourced and recyclable packaging
APPLICATIONS
PCR cleanup
DNA from PCR reactions can be purified after amplification to remove polymerases, primers, detergents, dNTPs, etc.
Enzymatic reaction cleanup
Restriction enzymes and modifying enzymes such as ligases, kinases, nucleases, phosphatases are efficiently removed, allowing for effective desalting and concentration of the DNA sample.
cDNA cleanup
DNA/RNA complexes can be purified post-reverse transcription/amplification to enable removal of the RT and polymerase as well as nucleotides.
Labeling cleanup
Unincorporated radiolabeled or fluorescently labeled nucleotides can be removed from the DNA substrate
Plasmid cleanup
Plasmid preps from unknown sources may contain inhibitors and unwanted contaminants. Purification and concentration can be easily achieved using this kit.
Oligonucleotide cleanup
ssDNA oligonucleotides (≥ 18 nt) and dsDNA fragments (≥ 15 bp) can be purified using the Oligonucleotide Cleanup Protocol.
Characteristics
DNA Sample Type:
DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations). ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol.
Binding Capacity:
up to 5 μg
DNA Size Range:
~50 bp to 25 kb DNA ≥ 15 bp to 25 kb (dsDNA) and DNA ≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol.
Typical Recovery:
DNA (50 bp to 10 kb): 70–90% DNA (11–23 kb): 50–70% ssDNA ≥ 18 nt and dsDNA ≥ 15 bp: 70-85%
Elution Volume:
≥ 6 μl
Purity:
A260/280 > 1.8 and A260/230 > 1.8
Protocol Time:
5 minutes of spin and incubation time
Compatible Downstream Applications:
ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing