T1030L, Monarch PCR & DNA Cleanup Kit (5ug) - 250 preps

APPLICATIONS

PCR cleanup

DNA from PCR reactions can be purified after amplification to remove polymerases, primers, detergents, dNTPs, etc.

Enzymatic reaction cleanup

Restriction enzymes and modifying enzymes such as ligases, kinases, nucleases, phosphatases are efficiently removed, allowing for effective desalting and concentration of the DNA sample.

cDNA cleanup

DNA/RNA complexes can be purified post-reverse transcription/amplification to enable removal of the RT and polymerase as well as nucleotides.

Labeling cleanup

Unincorporated radiolabeled or fluorescently labeled nucleotides can be removed from the DNA substrate

Plasmid cleanup

Plasmid preps from unknown sources may contain inhibitors and unwanted contaminants. Purification and concentration can be easily achieved using this kit.

Oligonucleotide cleanup

ssDNA oligonucleotides (≥ 18 nt) and dsDNA fragments (≥ 15 bp) can be purified using the Oligonucleotide Cleanup Protocol.

Characteristics

DNA Sample Type:

DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations).
ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol.

Binding Capacity:

up to 5 μg

DNA Size Range:

~50 bp to 25 kb 
DNA ≥  15 bp to 25 kb (dsDNA) and DNA ≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol.

Typical Recovery:

DNA (50 bp to 10 kb): 70–90%
DNA (11–23 kb): 50–70%
ssDNA ≥  18 nt and dsDNA ≥ 15 bp: 70-85%

Elution Volume:

≥ 6 μl

Purity:

A260/280 > 1.8 and A260/230 > 1.8

Protocol Time:

5 minutes of spin and incubation time

Compatible Downstream
Applications:

ligation, restriction digestion, labeling and other enzymatic
manipulations, library construction and DNA sequencing