S9221S, CLIP-Biotin - 50 nmol

CLIP-Biotin is suitable for applications such as biotinylation of CLIP-tag™ fusion proteins in living cells for detection with streptavidin fluorophore conjugates or labeling in solution for analysis by SDS-PAGE/Western blot or for capture with streptavidin for binding and interaction studies.

• It is a cell-permeable substrate (BC-Biotin)
• It is based on biotin with an amidocaproyl linker

This package contains 50 nmol of CLIP-Biotin substrate, sufficient to make 10 ml of a 5 μM solution for the labeling of CLIP-tag fusion proteins in cells.

CLIP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a small protein based on mammalian O⁶-alkylguanine-DNA-alkyltransferase (AGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether link. Although CLIP-tag is based on the same protein as SNAP-tag^®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal simultaneous labeling.