S9219S, CLIP-Cell TMR-Star - 30 nmol

CLIP-Cell™ TMR-Star is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells, on cell surfaces or in vitro.

• This is a cell-permeable substrate (BC-TMR) based on tetramethylrhodamine
• It is suitable for standard rhodamine filter sets
• It has an excitation maximum at 554 nm and emission maximum at 580 nm

This package includes 30 nmol of CLIP-Cell TMR-Star, sufficient to make 10 ml of a 3 µM CLIP-tag fusion protein labeling solution.

The CLIP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O⁶-alkylguanine-DNAalkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond.  Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.