S1420S, Streptavidin Magnetic Beads(20 mg) - 5 ml

Streptavidin Magnetic Beads are 1 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids.

• The strength of the biotin-streptavidin interaction, an association constant (Ka) of 1015 M-1, coupled with the low non-specific binding of streptavidin permits captured substrates to be useful as ligands in subsequent experiments including mRNA isolation, nucleic acid enrichments and the capture of primary or secondary antibodies
• Other applications include 5-hmC detection and analysis, methylated DNA analysis, and solution-phase panning in phage display experiments
• Binding capacity:  ≥30 µg biotinylated antibody per mg and >500 pmol of single-stranded 25 bp biotinylated oligionucleotide per mg
• High binding capacity and low non-specific binding of proteins make this bead ideally suited for protein isolation applications
• Quick, small-scale purification of biotinylated proteins affords higher throughput and efficiency

Properties & Usage

Storage Buffer

0.1% BSA
0.05% Tween® 20
0.05% NaN₃
1X PBS
pH 7.4 @ 25°C

Support Matrix: 1 µM nonporous superparamagnetic microparticle

Binding Capacity: The beads will bind greater than 1000 pmol of free biotin per mg and greater than 500 pmol of single-stranded 25 bp biotinylated oligionucleotide per mg.

Shipping Notes: 4°C