S1419S, Oligo d(T)25 Magnetic Beads - 5 ml

Oligo d(T)25 is covalently coupled to 1 μm superparamagnetic particle through a linkage that is stable and leak resistant over a wide pH range, thereby permitting the small-scale isolation of mRNA from crude cell lysates and tissue. The isolation occurs through the hybridization of covalently coupled oligo d(T)25 to the poly(A) region present in most eukaryotic mRNAs. 

• Allows for direct mRNA isolation following lysis and second-round purification of previously isolated total RNA
• The magnetic separation technology is scalable and permits elution of intact mRNA in small volumes, thereby eliminating the need for precipitation of the isolated mRNA
• Beads can be reused up to three times 
• The isolated mRNA can either be eluted or the bound d(T)25 can be used as a primer in a first-strand cDNA reaction

Supplied as a 5 mg/ml suspension in phosphate buffered saline (PBS buffer) (pH 7.4), containing 0.05% Tween^® 20 and 0.05% NaN₃.

Recommended Buffers

Lysis/Binding Buffer: 100 mM Tris-HCl, pH 7.5, 500 mM LiCl, 0.5% Lithium Dodecyl Sulfate (LiDS), 1 mM EDTA, 5 mM DTT

Wash Buffer I: 20 mM Tris-HCl, pH 7.5, 500 mM LiCl, 0.1% LiDS, 1 mM EDTA, 5 mM DTT

Wash Buffer II: 20 mM Tris-HCl, pH 7.5, 500 mM LiCl, 1 mM EDTA

Low-Salt Buffer: 20 mM Tris-HCl, pH 7.5, 200 mM LiCl, 1 mM EDTA

Elution Buffer: 20 mM Tris-HCl, pH 7.5, 1 mM EDTA