P0709S, PNGase F (Glycerol Free), recombinant - 15.000 units

PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F (Glycerol-free), Recombinant is a recombinant amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides. 

• Recombinant enzyme; guaranteed endoglycosidase F1, F2 or F3 free
• Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
• ≥ 95% purity, as determined by SDS-PAGE and intact ESI-MS
• Optimal activity and stability for up to 24 months
• Can be used under native or denaturing conditions
• Optimized for deglycosylation of glycoproteins; leaves N-glycan core oligosaccharides intact and suitable for further analysis

Product Source

Cloned from Elizabethkingia miricola (formerly Flavobacterium meningosepticum) and expressed in E. coli.